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dNTPs

 

Our ultra-pure dNTPs are certified free of nucleases and phosphatases. The functional purity is tested in Sanger dideoxy sequencing reactions. It is also tested by filling-in of 5′ overhangs and primer extensions using Klenow and several thermostable DNA polymerases. The chemical purity of this preparation is determined by HPLC, requiring a minimum passing specification of 99% triphosphate. Nucleotide concentrations are determined by measurements of absorbance. Each nucleotide is supplied at 100mM/40µmol in sterile, doubly distilled water. Dilutions of these nucleotides can be made using sterile, doubly distilled water.

 

Catalog Number Item Size Price
103 dATP (100 mM) 40 µmol (400 ml) $42.00
104 dCTP (100 mM) 40 µmol (400 ml) $42.00
105 dGTP (100 mM) 40 µmol (400 ml) $42.00
106 dTTP (100 mM) 40 µmol (400 ml) $42.00
107 dNTP Set (100 mM each) 4 x 40 µmol (4 x 400 ml) $149.00
116 dNTP mix (10 mM x 4) 500 ul   $39.00

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